Senior Scientist

2 tygodni temu

Kraków, Lesser Poland Selvita Group Pełny etat
Senior Scientist - Genetic Engineering/ Cell Line Production

Selvita is a global integrated drug discovery partner for the pharmaceutical and biotechnology industries with offices in Cambridge, UK, Greater Boston Area, San Francisco Bay Area, as well as, Krakow and Poznan. The company offers drug discovery support at every stage of the early discovery phase up to the preclinical research phase.

Selvita as a global CRO is matching its recruitment needs due to the growth of the project portfolio we perform for our clients. We are open for candidates with various professional experience and skillset however, final recruitment decisions will be based on current project needs.

As a Scientist in the field of Molecular Biology and Genetic Engineering/ Cell Line Production, you will play a crucial role in the development of tools and assays requiring introduction of genetic changes of various nature (e.g. gene knock-out, silencing, gene overexpression). The developed tools and assays will be used in the Drug Discovery process and products in the GMP environment for biotech and pharma companies from Europe, USA and the rest of the world.

You'll be working in a friendly and collaborative environment created by open-minded people, always ready for new challenges that new projects can bring, with an everyday opportunity to exchange knowledge with experienced members of the team.

Key Responsibilities

  • Molecular cloning (design and conduct of the cloning – e.g. bacteria transformation, plasmid amplification and isolation, colony PCR).
  • Genome engineering – design and cloning of gRNA.
  • Production of lentiviral vectors.
  • Culture of eukaryotic cell lines and cell line banking.
  • Introduction of RNA/DNA/protein into the eukaryotic cell lines (chemical transfection, electroporation, transduction with viral vectors).
  • Gene silencing through siRNA.
  • Designing of primers for PCR and qPCR.
  • Conducting PCR, qPCR and RT-PCR (experiment design, reaction setup, isolation of genomic DNA, isolation of RNA).
  • RNA/DNA electrophoresis.
  • Isolation of protein, protein electrophoresis, and Western blotting.
  • Flow cytometry (design and conduct of an entire experiment).
  • Proactively seeking out new information in the literature and incorporating this into individual projects as well as the overall program.
  • Presenting research results and methodologies at weekly meetings with the immediate supervisor and project leader, project team meetings, and other research forums.
  • Mentoring and supervising junior team members by providing scientific expertise and sharing technical skills.
Your Background
  • PhD with preferably 2+ years of experience.
  • Prior industrial or relevant academic experience.
  • Previous experience in the fields of molecular biology.
  • Previous experience in the field of genome engineering (e.g. CRISPR/Cas9, TALEN, ZFN).
  • Experience in the in vitro eukaryotic cell line culture as well as in chemical transfection and viral transduction.
  • Experience in working with bacteria (transformation, amplification etc.).
  • Very good knowledge and experience in molecular cloning techniques (design and conduct of the entire procedure).
  • Experience in working with DNA, RNA, and proteins.
  • Technical expertise with standard molecular biology techniques like ELISA, Western Blot, qPCR, as well as cytotoxicity, proliferation, apoptotic, and cellular reporter system assays.
  • Expertise with flow cytometry, cytokine release, cytotoxicity and proliferation assays is strongly preferred.
  • Experience in working with cell lines and cell-based assays in microplate format (experience with 3D assays will be a plus).
  • Expertise in working in GxP environment is preferred.
  • Practical experience with analysis software such as FlowJo, GraphPad Prism, SnapGene, and Excel is strongly preferred.
  • Ability to manage large amounts of data and to supervise less experienced scientists/research assistants.
  • Willingness to learn and adopt new experimental techniques.
  • Active participation in task performance and problem-solving attitude.
  • Good command of spoken and written English.
Your Benefits Package
  • Extensive research facilities and modern laboratories.
  • Daily cooperation and know-how exchange with an experienced research team.
  • Long-term cooperation based on stable employment conditions and a clear career path.
  • Support of experienced mentors with international scientific experience.
  • Benefits: private medical care, on-site fitness zone & subsidized sports card, lunch benefits & fruits in the office, on-site, subsidized kindergarten.
  • Support in legalization and relocation from abroad.
  • Language courses (English & Polish).
  • Complex training programs.
  • Support & incentive bonus for completing Ph.D.
  • Various sports initiatives.
  • Referral program.
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